One common microorganism used for this kind of biotechnology is called the pseudomonas putida p. The organism and its derivatives were grown in lb medium or in a defined medium mmy that contained, per liter, 5. The role of pseudomonas putida in bioremediation of naphthalene and copper article pdf available january 2011 with 966 reads how we measure reads. The objective of present study was to bioremediate cr vi in industrial effluent by using pseudomonas putida mtcc 102. Adaptive expansion of biodegradation by pseudomonas putida f1. Engineering bacteria for bioremediation dietmar h pieper and. Pseudomonas putida, a bacterium that colonizes plant roots and enhances plant growth, produces three isozymes of catalase a, b, and c in stationaryphase cells. Genomic features of pseudomonas putida strain kt2440. The refined analysis of poxn01 taxonomic affiliation was performed as above and included 20 strains of pseudomonas putida, 8 other species belonging to the p.
A pseudomonas putida strain genetically engineered for 1,2. A combination of physiological and biochemical assays, a genomewide collection of mutants and several omics approaches have provided useful information to help identify functions involved in solvent tolerance in p. Pseudomonas putida is a soil and water organism that uses a wide variety of organic compounds as carbon and energy sources. The removal rate of nb within 10 days, 6 mgkg and 53 mgl nitrobenzene in sediment and water, respectively, was degraded with the augmentation of pseudomonas putida isolated from the contaminated sediment. Pdf survival of pseudomonas putida as bioremediation agent in. Pseudomonas 3,17,2225, alcaligenes 26 and rhodococcus 5,27. Bioremediation have been investigated as alternative tools for residues oil clean up. We describe recovery from 10,941 samples collected during up to 8 visits.
Bioremediation of pnitrophenol by pseudomonas putida 1274 strain. Previous studies revealed that pseudomonas putida s16 is a nonpathogenic member of the genus pseudomonas, and utilizes a variety of organic wastes as a sole source of carbon andor nitrogen wang et al. Plasmid nah7 in pseudomonas putida g7 used to encode bacterial. Analysis of germination and growth ability the crude oil degrading ability of isolate. Spill simulations with biodiesel, diesel and their blends in sandy gravel soil were performed according to previous study with some modification. The commercialization of biodieseldiesel blends on the market can cause environmental damages due to spills. The present report of the genome sequence, coupled with an extensive analysis of p. Pdf over the past decade poly aromatic hydrocarbons and heavy metal compounds caused serious problems for marine environments. The physicochemical parameters of the soil selected for field experiments were also characterized. After two months of bioremediation treatment with pseudomonas putida strains, the concentration of naphthalene decreased by 63.
To date, no natural tcp degraders able to mineralize tcp aerobically have been isolated. Pseudomonas putida cbf102 is a microorganism isolated from farmland soil in fairchild, tx, found to degrade highimpact xenobiotics, including organophosphate insecticides, petroleum hydrocarbons, and both monocyclic and polycyclic aromatics. A candidate genome for genetic bioaugmentation vasundhara paliwal 0 sajan c. Bioremediation of pnitrophenol by pseudomonas putida 1274. Epidemiologic notes and reports reported contamination of. Pseudomonas putida is a gramnegative, rodshaped bacteria which undergoes aerobic metabolism. This bacterium was characterized on the basis of microbiological, biochemical and molecular analysis. Pdf the role of pseudomonas putida in bioremediation of. Bacterial growth optimization was studied based on carbon source, nitrogen. The host organism used in this study was pseudomonas putida strain mc4, a 2,3dichloropropanoldegrading bacterium. Pseudomonas putida and bacillus cereus were estimated by itoh and suzuki 1972 method. With highthroughput genetic techniques, we aim to improve the acid tolerance of a pollutantdegrading bacterium, pseudomonas putida s16 by. It plays a very important role in the decomposition that drives the carbon cycle. Inoculation of canola, tomato, and other agriculturally important plants with this strain results in substantial promotion of seedling root growth 7, 18.
A pseudomonas putida strain genetically engineered for 1,2,3trichloropropane bioremediation. The rhizobacterium pseudomonas putida gr122 is a strong candidate for development as a soil inoculant to enhance crop yields. Degradation, phytoprotection and phytoremediation of. Survival of pseudomonas putida for biodiesel blend b5 in. Paliwal v, raju sc, modak a, phale ps, purohit hj pseudomonas putida csv86. Pseudomonas putida and pseudomonas fluorescens species. Environmental microbes harbor an enormous pool of antibiotic and biocide resistance genes that can impact the resistance profiles of animal and human pathogens via horizontal gene transfer. Combinatorial metabolic engineering of pseudomonas putida.
Chromatereducing properties of soluble flavoproteins from. Melvin s samuel, akella sivaramakrishna, and alka mehta. Pseudomonas putida strains contain a circular chromosome of approximately 6 mbp which encodes about 5300 genes. Production of selenium nanoparticles in pseudomonas putida. Enhancing bioremediation potential of pseudomonas putida. Bioremediation is a complex process, with biological degradation taking place in the cells. This study aims to investigate the survival of pseudomonas putida p. Bioremediation of biofuelsoil contamination by using. The effectiveness on bioremediation have been conducted by examined several physicochemical tests on biodieseldiesel. Engineering pseudomonas putida kt2440 for simultaneous.
Pseudomonas putida strains are rapidly growing bacteria, frequently isolated from most temperate soils and waters, particularly polluted soils. The versatility of cbf102 makes it useful for multipurpose bioremediation of contaminated sites in agricultural and industrial environments. Nitrogen removal characteristics of pseudomonas putida y9. The investigated results were presented in table 8 and figure 5. The soilcontaminated areas can be cleanedup by the emerging science and technology of bioremediation. View pseudomonas putida research papers on academia. Pdf pseudomonas in biodegradation shivendra sharma. The members of the genus demonstrate a great deal of metabolic diversity and consequently are able to colonize a wide range of niches. Pseudomonas putida f1 ppf1 catabolizes aromatic compounds via benzoate dioxygenase, phenylacetylcoa epoxidase, pcymenemonooxygenase, and toluene dioxygenasemediated pathways, and the latter is shown here to be highly flexible, supporting growth on previously untested aromatic alkenes, esters, amides, alcohols, amines, and multiring compounds. Pseudomonas putida was isolated from the oilcontaminated sites of bhel raghavan and vivekanandan, 1999 and its pure culture was seeded, with and without concurrent application of fertilizers as a soil amendment. On three occasions, from december 1985 to january 1986, pseudomonas putida was isolated from routine surveillance cultures of bone marrow harvested from three donors at. Pseudomonas putida definition of pseudomonas putida by. Opmb efflux systems are involved in pyoverdine secretion in pseudomonas putida kt2440.
But pseudomonas putida removes maximum of 90% cr vi. Mechanisms of solvent resistance mediated by interplay of. Bioremediation is a process used to treat contaminated media, including water, soil and subsurface material, by altering environmental conditions to stimulate growth of microorganisms and degrade the target pollutants. Biological treatment is a similar approach used to treat wastes. A newly isolated strain pseudomonas fluorescens accession number kf 279042. Pseudomonas putida is a gramnegative, rodshaped, saprotrophic soil bacterium. Epidemiologic notes and reports reported contamination of heparin sodium with pseudomonas putida.
By shedding light on variation in time as well as in space, longterm biogeographic studies can help us define organisms distribution patterns and understand their underlying drivers. The maximum level of biosurfactants was noted in pseudomonas putida than bacillus cereus. Pseudomonas putida an overview sciencedirect topics. Antibiotic resistance determinants in a pseudomonas putida. Enhancement of hydrocarbons degradation by use of pseudomonas biosurfactants and biofilms amina meliani1 and ahmed bensoltane2 1laboratory of research of biological systems and geomatic, department of biology, faculty of science, university mascara, 29000, mascara, algeria 2laboratory of food and industrial microbiology, department of biology, faculty of science, university oran essenia. Here we examine distributions of pseudomonas in and around 15 human homes, focusing on the p. Pseudomonas putida strains are ubiquitous in soil and water but have been seldom isolated from humans. Material and methods for the bioremediation of cr vi the. The eflkctiveness on bioremediation have been conducted by examined several physicochemical tests on biodieselldieselcontaminated soil before and after seeding of p. Transformation of pseudomonas putida and escherichia coli with plasmidlinked drugresistance factor dna.
Pseudomonas putida is a bacteria, and is a very common germ that lives in soil and freshwater environments all over the world. Pseudomonas putida strain gj31 containing a novel chlorocatechol. In many cases, bioremediation is less expensive and more sustainable than other remediation alternatives. It is normally found in terrestrial and aquatic environments where oxygen is abundant. Isolation and molecular characterization of a novel. In this work, the chromate reductase activities of two electrophoretically pure soluble bacterial flavoproteinschrr from pseudomonas putida and yief from escherichia coliwere examined. This study aims to investigate the effectiveness of pseudomonas putida as oilbiodegradable agent in soil contaminated with biodiesevdiesel blend b20.
Aldehyde dehydrogenase diversity in bacteria of the pseudomonas genus. Onn malaysia uthm 86400, pant raja, batu pahat, johor, malaysia. Survival of pseudomonas putida as bioremediation agent in soil contaminated with biodieseldiesel mixture b50 blends. Natural attenuation, biostimulation and bioaugmentation of nitrobenzene nb contaminated sediments were investigated and compared. The study used burnt soil, which contains a high concentration of pahs, to study pseudomonas putida bioremediation capability. Proceedings of the national academy of sciences of the united states of america 72 9. On the basis of specialized techniques, hydroquinone was. Due to strong degradation ability in less incubation time and adaptability to temperature variation, strain p. The strain capable of heterotrophic nitrification and aerobic denitrification could efficiently remove ammonium, nitrate, and nitrite at an average removal rate of 2.
Bioremediation of nitrobenzenepolluted sediments by. Draft genome sequence of pseudomonas putida cbf102, a. This study was carried out in search of more efficient p. This study aims to investigate the effectiveness of pseudomonas putida as oilbiodegradable agent in soil contaminated with biodieseldiesel blend b20. Its broad substrate toluene dioxygenase has been widely utilized in biocatalytic syntheses of chiral chemicals, as well as in the metabolism and detoxification of tce, and in the production of indigo from. Aromatic and hydroaromatic compounds that are metabolized through the ketoadipate catabolic pathway serve as chemoattractants for pseudomonas putida f1. A pseudomonas putida strain genetically engineered for 1,2,3.
They are nutritional opportunists par excellence and a paradigm of metabolically versatile microorganisms that recycle organic wastes in aerobic and microaerophilic compartments of the environment, and that plays a key role in the maintenance. The effectiveness of bioremediation treatment for dieselsoil. An industrial waste, 1,2,3trichloropropane tcp, is toxic and extremely recalcitrant to biodegradation. Such enzymes are not appropriate for bioremediation, as they harm the bacteria and their primary end product is not criii.
Bioremediation of oilspilled sites through seeding of. Pseudomonas is a genus of gramnegative, gammaproteobacteria, belonging to the family pseudomonadaceae and containing 191 validly described species. Pseudomonas putidas role in the bioremediation of plastic. Briefly, 200 ml of pseudomonas putida was inoculated into soil samples. Pseudomonas putida one common microorganism used for this kind of biotechnology is called the pseudomonas putida p. Influence of oil contamination levels on hydrocarbon biodegradation in sandy sediment. Biodegradation of petroleum and crude oil by pseudomonas putida. Their ease of culture in vitro and availability of an increasing number of pseudomonas strain. The spillage simulation of b5 was conducted at laboratory scale for 24 days of treatment. Most species are saprophytic, but some are pathogenic for plants and animals.
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